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Checkpoint kinase 1 negatively regulates somatic hypermutation.
Nucleic Acids Res. 42, 3666-3674 (2014)
Immunoglobulin (Ig) diversification by somatic hypermutation in germinal center B cells is instrumental for maturation of the humoral immune response, but also bears the risk of excessive or aberrant genetic changes. Thus, introduction of DNA damage by activation-induced cytidine deaminase as well as DNA repair by multiple pathways need to be tightly regulated during the germinal center response to prevent lymphomagenesis. In the present study, we show that DNA damage checkpoint signaling via checkpoint kinase 1 (Chk1) negatively regulates somatic hypermutation. Chk1 inhibition in human B cell lymphoma lines as well as inactivation of Chk1 alleles by gene targeting in DT40 B cells leads to increased somatic hypermutation. This is apparently due to changes in DNA repair pathways regulated by Chk1, such as a decreased homologous recombination efficiency that also leads to decreased Ig gene conversion in DT40. Our data show that Chk1 signaling plays a crucial role in regulation of Ig diversification and sheds unexpected light on potential origins of aberrant somatic hypermutation in B cell lymphomagenesis.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Induced Cytidine Deaminase; Immunoglobulin Gene Conversion; Center B-cells; Homologous Recombination; Dna-damage; Cycle; Chk1; Repair; Breaks; Radiation
Language
english
Publication Year
2014
HGF-reported in Year
2014
ISSN (print) / ISBN
0305-1048
e-ISSN
1362-4962
Journal
Nucleic Acids Research
Quellenangaben
Volume: 42,
Issue: 6,
Pages: 3666-3674
Publisher
Oxford University Press
Publishing Place
Oxford
Reviewing status
Peer reviewed
Institute(s)
Research Unit Gene Vector (AGV)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF-Topic(s)
30203 - Molecular Targets and Therapies
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s)
Immune Response and Infection
PSP Element(s)
G-501500-003
G-501400-001
G-501400-001
PubMed ID
24423870
WOS ID
WOS:000334758600025
Scopus ID
84898969703
Erfassungsdatum
2014-02-04