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Peters, D.M.* ; Vadász, I.* ; Wujak, L.* ; Wygrecka, M.* ; Olschewski, A.* ; Becker, C.* ; Herold, S.* ; Papp, R.* ; Mayer, K.* ; Rummel, S.* ; Brandes, R.P.* ; Günther, A.* ; Waldegger, S.* ; Eickelberg, O. ; Seeger, W.* ; Morty, R.E.*

TGF-β directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury.

Proc. Natl. Acad. Sci. U.S.A. 111, E374-E383 (2014)
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GF-β is a pathogenic factor in patients with acute respiratory distress syndrome (ARDS), a condition characterized by alveolar edema. A unique TGF-β pathway is described, which rapidly promoted internalization of the αβγ epithelial sodium channel (ENaC) complex from the alveolar epithelial cell surface, leading to persistence of pulmonary edema. TGF-β applied to the alveolar airspaces of live rabbits or isolated rabbit lungs blocked sodium transport and caused fluid retention, which-together with patch-clamp and flow cytometry studies-identified ENaC as the target of TGF-β. TGF-β rapidly and sequentially activated phospholipase D1, phosphatidylinositol-4-phosphate 5-kinase 1α, and NADPH oxidase 4 (NOX4) to produce reactive oxygen species, driving internalization of βENaC, the subunit responsible for cell-surface stability of the αβγENaC complex. ENaC internalization was dependent on oxidation of βENaC Cys(43). Treatment of alveolar epithelial cells with bronchoalveolar lavage fluids from ARDS patients drove βENaC internalization, which was inhibited by a TGF-β neutralizing antibody and a Tgfbr1 inhibitor. Pharmacological inhibition of TGF-β signaling in vivo in mice, and genetic ablation of the nox4 gene in mice, protected against perturbed lung fluid balance in a bleomycin model of lung injury, highlighting a role for both proximal and distal components of this unique ENaC regulatory pathway in lung fluid balance. These data describe a unique TGF-β-dependent mechanism that regulates ion and fluid transport in the lung, which is not only relevant to the pathological mechanisms of ARDS, but might also represent a physiological means of acutely regulating ENaC activity in the lung and other organs.
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Publication type Article: Journal article
Document type Scientific Article
Keywords alveolar epithelium; fluid homeostasis; Respiratory-distress-syndrome; Ubiquitin Ligase Nedd4l; Cell-surface Expression; Transforming Growth-factor-beta-1; Na+ Channel; Reactive Oxygen; Molecular Characterization; Biophysical Properties; Nadph Oxidases; Disease
Language english
Publication Year 2014
Prepublished in Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 111, Issue: 3, Pages: E374-E383 Article Number: , Supplement: ,
Publisher National Academy of Sciences
Reviewing status Peer reviewed
Institute(s) Institute of Lung Health and Immunity (LHI)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Lung Research
PSP Element(s) G-501600-001
G-505000-006
PubMed ID 24324142
DOI 10.1073/pnas.1306798111
WOS ID WOS:000329928400012
Erfassungsdatum 2013-12-31
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