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Open Access Green as soon as Postprint is submitted to ZB.
Generation of chromosome painting probes from single chromosomes by laser microdissection and linker-adaptor PCR.
Chromosome Res. 12, 337-343 (2004)
Fluorescence in situ hybridization (FISH) plays an essential role in research and clinical diagnostics. The versatility and resolution of FISH depends critically on the probe set used. Here, we describe an improved approach for the generation of specific DNA probes from single copies of chromosomes. Single chromosomes or single chromosomal regions were microdissected by laser pressure catapulting and amplified using linker-adaptor PCR. The probes were labeled and tested in various scenarios including multicolor-FISH experiments employing up to seven different fluorochromes. FISH confirmed the specific and even staining of the respective chromosomal regions. Furthermore, the capability of these probes to detect even small translocations (<3 Mb) suggests that the dissected regions are completely represented in the generated painting probes.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
chromosome painting; laser microdissection; linker-adaptor PCR; molecular cytogenetics
ISSN (print) / ISBN
0967-3849
e-ISSN
1573-6849
Journal
Chromosome Research
Quellenangaben
Volume: 12,
Issue: 4,
Pages: 337-343
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institute of Human Genetics (IHG)