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Dalke, C. ; Löster, J. ; Fuchs, H. ; Gailus-Durner, V. ; Soewarto, D. ; Favor, J. ; Neuhäuser-Klaus, A. ; Pretsch, W. ; Gekeler, F.* ; Shinoda, K.* ; Zrenner, E.* ; Meitinger, T. ; Hrabě de Angelis, M. ; Graw, J.

Electroretinography as a screening method for mutations causing retinal dysfunction in mice.

Invest. Ophthalmol. Vis. Sci. 45, 601-609 (2004)
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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
PURPOSE. To detect mice with hereditary retinal impairment, a high-throughput electroretinography (ERG) screening system was established. METHOD. Mice from eight different strains without known retinal disorders (102, 129/Svj, AKR, C57BL/6j, C5713L/6JIco, CBA/CaJ, and DBA/2NCrlBR) and one control strain with retinal degeneration (C3HeB/FcJ) were fixed on a specially constructed sled, ERG electrodes were placed on the cornea, and mice were moved into a Ganzfeld stimulator. From a luminance range of 0.0125 to 500 cd-s/m(2) in a pretest series two levels (5 and 125 cd-s/m(2)) were chosen to shorten examination times. The root mean square (RMS) of the ERG-recording was analyzed to detect animals with abnormal retinal function. ERG responses of the left and right eyes were compared in amplitudes and implicit times of the a- and b-waves. Statistical analysis of the latter parameters was performed in all wild-type animals. Histology was performed on selected mice. RESULTS. ERG recordings of individual animals for the left and right eye revealed good agreement in amplitudes and implicit times of the a- and b-waves (P < 0.05). Comparison of these parameters among the wild-type strains showed several differences. Evaluation of the RMS revealed, in addition to the C3HeB/FeJ mice, a subgroup of mice within the 129/Svj strain with abnormal retinal function. Molecular analysis of these mice demonstrated the presence of the same retroviral insertion in the Pde6b gene, which is causative of the Pde6b(rd1) allele carried in C3HeB/FeJ mice. Histologic analysis demonstrated good correlation between retinal electrophysiology and morphology. CONCLUSIONS. The present results demonstrate the feasibility of ERG for screening a large number of mice to detect animals with functional retinal impairment.
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Publication type Article: Journal article
Document type Scientific Article
Keywords CONE-ROD DYSTROPHY; USHER-SYNDROME; RETINITIS-PIGMENTOSA; MACULAR DYSTROPHY; KNOCKOUT MICE; BETA-SUBUNIT; CGMP PHOSPHODIESTERASE; STARGARDTS-DISEASE; BINDING-PROTEIN; GATED CHANNEL
Language english
Publication Year 2004
HGF-reported in Year 2004
ISSN (print) / ISBN 0146-0404
e-ISSN 1552-5783
Journal Investigative Ophthalmology & Visual Science, IOVS
Quellenangaben Volume: 45, Issue: 2, Pages: 601-609 Article Number: , Supplement: ,
Publisher Association for Research in Vision and Ophthalmology (ARVO)
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)
Institute of Experimental Genetics (IEG)
Institute of Human Genetics (IHG)
POF-Topic(s) 30204 - Cell Programming and Repair
30201 - Metabolic Health
Research field(s) Genetics and Epidemiology
PSP Element(s) G-500500-002
G-500600-003
FE 70731
DOI 10.1167/iovs.03-0561
Scopus ID 9144242396
Erfassungsdatum 2004-03-12
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