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Bayer, C.* ; Liebhardt, M.E.* ; Schmid, T.E.* ; Trajkovic-Arsic, M.* ; Hube, K.* ; Specht, H.M.* ; Schilling, D. ; Gehrmann, M.* ; Stangl, S.* ; Siveke, J.T.* ; Wilkens, J.J.* ; Multhoff, G.

Validation of heat shock protein 70 as a tumor-specific biomarker for monitoring the outcome of radiation therapy in tumor mouse models.

Int. J. Radiat. Oncol. Biol. Phys. 88, 694-700 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
PURPOSE: Tumor cells, in contrast to normal cells, frequently overexpress heat shock protein 70 (Hsp70) in the cytosol, present it on their cell surface, and actively release it. Therefore, soluble Hsp70 (sHsp70) was investigated as a potential tumor biomarker for monitoring the outcome of radiation therapy. METHODS AND MATERIALS: Plasma from mice bearing membrane Hsp70 (mHsp70)-positive FaDu human squamous cell carcinoma of the head and neck and spontaneous pancreatic ductal adenocarcinoma (PDAC) was investigated. A cohort of mice with FaDu tumors (0.32 cm(3)) was irradiated with 30 Gy, and plasma was collected 24 hours after irradiation, after the tumors had shrunk to 50% of their starting volume and after complete remission. sHsp70 levels in the plasma were quantified by enzyme-linked immunosorbent assay. RESULTS: sHsp70 levels were significantly higher in the blood of tumor-bearing mice than that of control animals. A correlation between increasing sHsp70 plasma levels and tumor volume in the range of 0.01 cm(3) to 0.66 cm(3) was observed. Radiation-induced regression of the tumors was associated with significantly decreased sHsp70 levels, which returned to the level of control animals after complete remission. CONCLUSION: We propose sHsp70 as an innovative biomarker for detecting tumors and for monitoring the clinical outcome of radiation therapy in cancer patients.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Heat-shock Proteins; Natural-killer-cells; Lung-cancer Patients; Stress-proteins; Circulating Heat-shock-protein-70; Serum Hsp70; Surface; Autoantibody; Expression; Carcinoma
Language english
Publication Year 2014
HGF-reported in Year 2014
ISSN (print) / ISBN 0360-3016
e-ISSN 0360-3016
Journal International Journal of Radiation Oncology, Biology, Physics
Quellenangaben Volume: 88, Issue: 3, Pages: 694-700 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place New York
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-521400-001
PubMed ID 24521683
DOI 10.1016/j.ijrobp.2013.11.008
WOS ID WOS:000331726300026
Erfassungsdatum 2014-03-06
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