PuSH - Publication Server of Helmholtz Zentrum München: Unraveling the equine lymphocyte proteome: Differential septin 7 expression associates with immune cells in equine recurrent uveitis.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Degroote, R.L.* ; Hauck, S.M. ; Amann, B.* ; Hirmer, S.* ; Ueffing, M. ; Deeg, C.A.*

Unraveling the equine lymphocyte proteome: Differential septin 7 expression associates with immune cells in equine recurrent uveitis.

PLoS ONE 9:e91684 (2014)

Publ. Version/Full Text Volltext

DOI

PMC

	Open Access Gold

Abstract
Metrics
Extra information

Equine recurrent uveitis is a spontaneous, lymphocyte-driven autoimmune disease. It affects horses worldwide and presents with painful remitting-relapsing inflammatory attacks of inner eye structures eventually leading to blindness. Since lymphocytes are the key players in equine recurrent uveitis, we were interested in potential changes of their protein repertoire which may be involved in disease pathogenesis. To create a reference for differential proteome analysis, we first unraveled the equine lymphocyte proteome by two-dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis and subsequently identified 352 protein spots. Next, we compared lymphocytes from ERU cases and healthy horses with a two-dimensional fluorescence difference in gel electrophoresis approach. With this technique, we identified seven differentially expressed proteins between conditions. One of the significantly lower expressed candidates, septin 7, plays a role in regulation of cell shape, motility and migration. Further analyses revealed T cells as the main cell type with decreased septin 7 abundance in equine recurrent uveitis. These findings point to a possible pathogenetic role of septin 7 in this sight-threatening disease.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Blood Mononuclear-cells; Retinal S-antigen; Autoimmune Uveitis; Membrane-proteins; Mammalian Septins; Binding Protein; T-lymphocytes; Target Tissue; Identification; Autoantigens

ISSN (print) / ISBN 1932-6203

Journal PLoS ONE

Quellenangaben Volume: 9, Issue: 3, Article Number: e91684

Publisher Public Library of Science (PLoS)

Publishing Place Lawrence, Kan.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CF Metabolomics & Proteomics (CF-MPC)