PuSH - Publication Server of Helmholtz Zentrum München

Schlicke, H.* ; Hartwig, A.S.* ; Firtzlaff, V.* ; Richter, A.S.* ; Glässer, C.* ; Mayer, K.F.X. ; Finkemeier, I.* ; Grimm, B.*

Induced deactivation of genes encoding chlorophyll biosynthesis enzymes disentangles tetrapyrrole-mediated retrograde signalling.

Mol. Plant 7, 1211-1227 (2014)
Publ. Version/Full Text Volltext DOI PMC
Closed
Open Access Green as soon as Postprint is submitted to ZB.
In photosynthetic organisms, tetrapyrrole-mediated retrograde signals are proposed to contribute to a balanced nuclear gene expression (NGE) in response to metabolic activity in chloroplasts. We followed an experimental short-term approach that allowed the assessment of modified NGE during the first hours of specifically modified enzymatic steps of the Mg branch of tetrapyrrole biosynthesis, when pleiotropic effects of other signals can be avoided. In response to 24 h-induced silencing of CHLH, CHLM and CHL27 encoding the CHLH subunit of Mg chelatase, the Mg protoporphyrin methyltransferase and Mg protoporphyrin monomethylester cyclase, respectively, deactivated gene expression rapidly led to reduced activity of the corresponding enzymes and altered Mg porphyrin levels. But NGE was not substantially altered. When these three genes were continuously inactivated for up to 4 days, changes of transcript levels of nuclear genes were determined. CHL27 silencing for more than 24 h results in necrotic leaf lesions and modulated transcript levels of oxidative stress-responsive and photosynthesis-associated nuclear genes (PhANGs). The prolonged deactivation of CHLH and CHLM results in slightly elevated transcript levels of PhANGs and tetrapyrrole-associated genes. These time-resolved studies indicate a complex scenario for the contribution of tetrapyrrole biosynthesis on NGE mediated by (1)O2-induced signalling and feedback-regulated ALA synthesis.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Mg Protoporphyrin ; Plastid Signal ; Retrograde Signal ; Tetrapyrrole Metabolism ; Transcription Analysis; Mg-protoporphyrin-ix; Singlet Oxygen; Arabidopsis-thaliana; Chloroplast Development; Aminolevulinic-acid; Oxidative Stress; Plastid Signals; Redox Signals; De-etiolation; Nuclear Genes
ISSN (print) / ISBN 1674-2052
e-ISSN 1752-9867
Journal Molecular Plant
Quellenangaben Volume: 7, Issue: 7, Pages: 1211-1227 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Oxford
Reviewing status Peer reviewed
Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)