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Open Access Green as soon as Postprint is submitted to ZB.
The intra viral protein interaction network of hepatitis C virus.
Mol. Cell. Proteomics 13, 1676-1689 (2014)
Hepatitis C virus (HCV) is a global health problem and one of the main reasons for chronic liver disease like cirrhosis and hepatocellular carcinoma. The HCV genome is translated into a polyprotein which is proteolytically processed into ten viral proteins. The interactome of the HCV proteins with the host cell has been worked out, however it remains unclear how viral proteins interact with each other. We aimed to generate the interaction network of these ten HCV proteins by a flow cytometry based FRET assay established in our laboratory (Banning et al, 2010 PLoS ONE 5(2): e9344). HCV proteins were constructed as fusions with the chromophores CFP and YFP. All HCV fusions were expressed and localized to specific subcellular compartments, indicating that they are functional. FACS-FRET measurements identified a total of 20 interactions. 13 of these were previously described and are now confirmed by our method in living cells. Among the seven novel protein binding pairs HCV p7 plays a pivotal role. It binds to the HCV capsid protein Core and the two glycoproteins E1 and E2. These interplays were further demonstrated in the relevant context of Huh7.5 liver cells expressing infectious HCV. Our work demonstrates the feasibility to rapidly generate small interaction networks by FACS-FRET and defines the network of intra HCV protein interactions. Furthermore, our data supports an important role of p7 in HCV assembly.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Fret/flim ; Hepatitis C Virus ; Host-pathogen Interaction ; Infectious Disease ; Protein-protein Interactions* ; Viruses; Dependent Rna-polymerase; Core Protein; Nonstructural Protein; Complex-formation; Infectious Virus; Ns3 Helicase; Ion-channel; Recombinant Vaccinia; Genetic Interaction; Crystal-structure
ISSN (print) / ISBN
1535-9476
e-ISSN
1535-9484
Quellenangaben
Volume: 13,
Issue: 7,
Pages: 1676-1689
Publisher
American Society for Biochemistry and Molecular Biology
Publishing Place
Bethesda
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)