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Brockmann, A.* ; Strittmatter, T.* ; May, S.* ; Stemmer, K. ; Marx, A.* ; Brunner, T.*

Structure-function relationship of thiazolide-induced apoptosis in colorectal tumor cells.

ACS Chem. Biol. 9, 1520-1527 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Thiazolides are a novel class of anti-infectious agents against intestinal intracellular and extracellular protozoan parasites, bacteria, and viruses. While the parent compound nitazoxanide (NTZ; 2-(acetolyloxy)-N-(5-nitro-2-thiazolyl)benzamide) has potent antimicrobial activity, the bromo-thiazolide RM4819 (N-(5-bromothiazol-2-yl)-2-hydroxy-3-methylbenzamide) shows only reduced activity. Interestingly, both molecules are able to induce cell death in colon carcinoma cell lines, indicating that the molecular target in intestinal pathogens and in colon cancer cells is different. The detoxification enzyme glutathione S-transferase of class Pi 1 (GSTP1) is frequently overexpressed in various tumors, including colon carcinomas, and limits the efficacy of antitumor chemotherapeutic drugs due to its detoxifying activities. In colorectal tumor cells RM4819 has been shown to interact with GSTP1, and GSTP1 enzymatic activity is required for thiazolide-induced apoptosis. At present it is unclear which molecular structures of RM4819 are required to interact with GSTP1 and to induce cell death in colon carcinoma cell lines. Here, we demonstrate that novel thiazolide derivatives with variation in their substituents of the benzene ring do not significantly affect apoptosis induction in Caco-2 cells, whereas removal of the bromide atom on the thiazole ring leads to a strong reduction of cell death induction in colon cancer cells. We further show that active thiazolides require caspase activation and GSTP1 expression in order to induce apoptosis. We demonstrate that increased glutathione (GSH) levels sensitize colon cancer cells to thiazolides, indicating that both GSTP1 enzymatic activity as well as GSH levels are critical factors in thiazolide-induced cell death.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Glutathione-s-transferase; Anticancer Drug-resistance; Cancer-cells; Nitazoxanide; Death; Pi; Nomenclature; Metabolism; Activation; Giardiasis
ISSN (print) / ISBN 1554-8929
e-ISSN 1554-8937
Journal ACS Chemical Biology
Quellenangaben Volume: 9, Issue: 7, Pages: 1520-1527 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place Washington
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Obesity (IDO)