PuSH - Publication Server of Helmholtz Zentrum München

Hartl, D.* ; Koller, B. ; Mehlhorn, A.T.* ; Reinhardt, D.* ; Nicolai, T.* ; Schendel, D.J. ; Griese, M.* ; Krauss-Etschmann, S.

Quantitative and functional impairment of pulmonary CD4⁺CD25hi regulatory T cells in pediatric asthma.

J. Allergy Clin. Immunol. 119, 1258-1266 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Asthma is characterized by a T(H)2 immune response. CD4(+)CD25(hi) regulatory T cells (Tregs) have been proposed to prevent allergic diseases through suppression of T(H)2 responses. OBJECTIVE: We sought to investigate the role of CD4(+)CD25(hi) T cells in children with asthma. METHODS: CD4(+)CD25(hi) Tregs and forkhead/winged-helix transcription factor FOXP3 mRNA levels were quantified in peripheral blood and bronchoalveolar lavage fluid (BALF) of 18 children with asthma, 10 children with chronic cough, and 13 control subjects without lung diseases. CD4(+)CD25(hi) T cells were isolated from peripheral blood and BALF of asthmatic patients and control subjects, and their capacity to suppress proliferation and cytokine/chemokine production of autologous responder T cells was analyzed. RESULTS: CD4(+)CD25(hi) T cells were decreased in BALF of asthmatic children compared with values in children with cough or control subjects. In children with asthma, inhaled corticosteroid treatment was associated with increased percentages of CD4(+)CD25(hi) T cells in peripheral blood and BALF. Isolated BALF and peripheral blood CD4(+)CD25(hi) T cells from nonasthmatic subjects suppressed proliferation and cytokine/chemokine production by CD4(+)CD25(-) responder T cells. BALF CD4(+)CD25(hi) T cells from asthmatic subjects failed to suppress proliferation and production of T(H)2-associated cytokines and chemokines by CD4(+)CD25(-) responder T cells, which was restored after use of inhaled corticosteroids. CONCLUSION: These findings provide the first evidence that pulmonary CD4(+)CD25(hi) Tregs are impaired in pediatric asthma. CLINICAL IMPLICATIONS: Pulmonary Tregs might represent a therapeutic target in pediatric asthma.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Asthma; children; CD4+CD25hi cells; regulatory T cells; FOXP3; bronchoalveolar lavage
ISSN (print) / ISBN 0091-6749
e-ISSN 1097-6825
Journal The journal of allergy and clinical immunology
Quellenangaben Volume: 119, Issue: 5, Pages: 1258-1266 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam [u.a.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Immune Regulation in Childhood (IMI-KIK)