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Graef, P.* ; Buchholz, V.R.* ; Stemberger, C.* ; Flossdorf, M.* ; Henkel, L.* ; Schiemann, M. ; Drexler, I.* ; Höfer, T.* ; Riddell, S.R.* ; Busch, D.H.

Serial transfer of single-cell-derived immunocompetence reveals stemness of CD8+ central memory T cells.

Immunity 41, 116-126 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Maintenance of immunological memory has been proposed to rely on stem-cell-like lymphocytes. However, data supporting this hypothesis are focused on the developmental potential of lymphocyte populations and are thus insufficient to establish the functional hallmarks of stemness. Here, we investigated self-renewal capacity and multipotency of individual memory lymphocytes by in vivo fate mapping of CD8(+) T cells and their descendants across three generations of serial single-cell adoptive transfer and infection-driven re-expansion. We found that immune responses derived from single naive T (Tn) cells, single primary, and single secondary central memory T (Tcm) cells reached similar size and phenotypic diversity, were subjected to comparable stochastic variation, and could ultimately reconstitute immunocompetence against an otherwise lethal infection with the bacterial pathogen Listeria monocytogenes. These observations establish that adult tissue stem cells reside within the CD62L(+) Tcm cell compartment and highlight the promising therapeutic potential of this immune cell subset.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1074-7613
e-ISSN 1097-4180
Journal Immunity
Quellenangaben Volume: 41, Issue: 1, Pages: 116-126 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place Cambridge, Mass.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)
CCG Antigen-specific Immunotherapy (VIRO-KVA)