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HIV-1 replication in human immune cells is independent of TAR DNA binding Protein 43 (TDP-43) expression.
PLoS ONE 9:e105478 (2014)
The TAR DNA binding protein (TDP-43) was originally identified as a host cell factor binding to the HIV-1 LTR and thereby suppressing HIV-1 transcription and gene expression (Ou et al., J.Virol. 1995, 69(6):3584). TDP-43 is a global regulator of transcription, can influence RNA metabolism in many different ways and is ubiquitously expressed. Thus, TDP-43 could be a major factor restricting HIV-1 replication at the level of LTR transcription and gene expression. These facts prompted us to revisit the role of TDP-43 for HIV-1 replication. We utilized established HIV-1 cell culture systems as well as primary cell models and performed a comprehensive analysis of TDP-43 function and investigated its putative impact on HIV-1 gene expression. In HIV-1 infected cells TDP-43 was neither degraded nor sequestered from the nucleus. Furthermore, TDP-43 overexpression as well as siRNA mediated knockdown did not affect HIV-1 gene expression and virus production in T cells and macrophages. In summary, our experiments argue against a restricting role of TDP-43 during HIV-1 replication in immune cells.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Human-immunodeficiency-virus; T-cells; Restriction Factors; Nuclear Factor; Nef; Release; Rna; Vpu; Transcription; Macrophages
ISSN (print) / ISBN
1932-6203
Journal
PLoS ONE
Quellenangaben
Volume: 9,
Issue: 8,
Article Number: e105478
Publisher
Public Library of Science (PLoS)
Publishing Place
Lawrence, Kan.
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Reviewing status
Peer reviewed