PuSH - Publication Server of Helmholtz Zentrum München: Genetic analysis of DEK1 loop function in three-dimensional body patterning in physcomitrella patens.

Information

Clues to quality of journals

Open Access Policy of Helmholtz Association 2016

CC Licencences

Publication Metrics

Navigation

Home

Deutsch

EVA: Electronic Publishing

New EVA Application

Research

Advanced Search

Browse by ...

... HMGU-Authors/Consortia

... Organizational Structure

... Journal

... Publication Type

... Research Data

... Arbeitsgruppen

... Publication Year

Publication overview

Statistics

Statistics (last 5 years)

OA Publications

Publish

Submit Publication

Import publication from...

...EVA

Report missing publication

Highlights

Support & Contact

Contact persons

Help

Data protection

Helmholtz Open Science

JANE Journal Estimator

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Demko, V.* ; Perroud, P.F.* ; Johansen, W.* ; Delwiche, C.F.* ; Cooper, E.D.* ; Remme, P.* ; Ako, A.E.* ; Kugler, K.G. ; Mayer, K.F.X. ; Quatrano, R.S.* ; Olsen, O.A.*

Genetic analysis of DEK1 loop function in three-dimensional body patterning in physcomitrella patens.

Plant Physiol. 166, 903-919 (2014)

Publ. Version/Full Text

DOI

PMC

	Closed

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

DEK1 of higher plants plays an essential role in position dependent signaling and consists of a large transmembrane domain (MEM) linked to a protease catalytic domain (CysPc) and a regulatory domain (C2L). Here we show that the postulated sensory Loop of the MEM domain plays an important role in the developmental regulation of DEK1 activity in the moss Physcomitrella patens. Compared with P. patens lacking DEK1 (∆dek1), the dek1∆loop mutant correctly positions the division plane in the bud apical cell. In contrast to an early developmental arrest of ∆dek1 buds, dek1∆loop develops aberrant gametophores lacking expanded phyllids resulting from mis-regulation of mitotic activity. In contrast to the highly conserved sequence of the catalytic CysPc domain, the Loop is highly variable in land plants. Functionally, the sequence from Marchantia polymorpha fully complements the dek1∆loop phenotype, whereas sequences from Zea mays and Arabidopsis thaliana give phenotypes with retarded growth and affected phyllid development. New bioinformatic analysis identifies MEM as a member of the Major Facilitator Superfamily, membrane transporters reacting to stimuli from the external environment. Transcriptome analysis comparing WT and ∆dek1 tissues identifies an effect of two groups of transcripts connected to dek1 mutant phenotypes, i.e. transcripts related to cell wall remodeling and regulation of the APB2 and APB3 transcription factors known to regulate bud initiation. Finally, new sequence data support the hypothesis that the advanced charophyte algae that evolved into ancestral land plants lost cytosolic calpains, retaining DEK1 as the sole calpain in the evolving land plant lineage.

Impact Factor

Scopus SNIP

Web of Science
Times Cited

Scopus
Cited By

Altmetric

7.394

2.093