PuSH - Publication Server of Helmholtz Zentrum München: Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Tano, K.* ; Nakamura, J.* ; Asagoshi, K.* ; Arakawa, H. ; Sonoda, E.* ; Braithwaite, E.K.* ; Prasad, R.* ; Buerstedde, J.M. ; Takeda, S.* ; Watanabe, M.* ; Wilson, S.H.*

Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells.

DNA Repair 6, 869-875 (2007)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

DNA polymerase lambda (Pol lambda) is a DNA polymerase beta (Pol beta)-like enzyme with both DNA synthetic and 5'-deoxyribose-5'-phosphate lyase domains. Recent biochemical studies implicated Pol lambda as a backup enzyme to Pol beta in the mammalian base excision repair (BER) pathway. To examine the interrelationship between Pol lambda and Pol beta in BER of DNA damage in living cells, we disrupted the genes for both enzymes either singly or in combination in the chicken DT40 cell line and then characterized BER phenotypes. Disruption of the genes for both polymerases caused hypersensitivity to H(2)O(2)-induced cytotoxicity, whereas the effect of disruption of either polymerase alone was only modest. Similarly, BER capacity in cells after H(2)O(2) exposure was lower in Pol beta(-/-)/Pol lambda(-/-) cells than in Pol beta(-/-), wild-type, and Pol lambda(-/-) cells, which were equivalent. These results suggest that these polymerases can complement for one another in counteracting oxidative DNA damage. Similar results were obtained in assays for in vitro BER capacity using cell extracts. With MMS-induced cytotoxicity, there was no significant effect on either survival or BER capacity from Pol lambda gene disruption. A strong hypersensitivity and reduction in BER capacity was observed for Pol beta(-/-)/Pol lambda(-/-) and Pol beta(-/-) cells, suggesting that Pol beta had a dominant role in counteracting alkylation DNA damage in this cell system.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords DNA polymerase lambda; DNA polymerase ß; Oxidation DNA damage; DT40

ISSN (print) / ISBN 1568-7864

e-ISSN 1568-7856

Journal DNA Repair

Quellenangaben Volume: 6, Issue: 6, Pages: 869-875

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Molecular Radiation Biology (IMS)