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Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.
Analyst 139, 5709-5717 (2014)
Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Assisted-laser-desorption/ionization; Mass-spectrometry; Yeast-cells; Metabolomics; Heterogeneity; Matrix; Metabolism; Switch; Flux
ISSN (print) / ISBN
0003-2654
e-ISSN
1364-5528
Journal
Analyst, The
Quellenangaben
Volume: 139,
Issue: 22,
Pages: 5709-5717
Publisher
Royal Society of Chemistry (RSC)
Publishing Place
Cambridge
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Computational Biology (ICB)