PuSH - Publication Server of Helmholtz Zentrum München: Molecular phenotypic profiling of a <em>Saccharomyces cerevisiae</em> strain at the single-cell level.

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Schmidt, A.M.* ; Fagerer, S.R.* ; Jefimovs, K.* ; Buettner, F. ; Marro, C.* ; Siringil, E.C.* ; Boehlen, K.L.* ; Pabst, M.* ; Ibáñez, A.J.*

Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.

Analyst 139, 5709-5717 (2014)

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Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.

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