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Tomi, N.S.* ; Davari, K.* ; Grotzky, D. ; Loos, F. ; Böttcher, K.* ; Frankenberger, S. ; Jungnickel, B.*

Analysis of SHPRH functions in DNA repair and immunoglobulin diversification.

DNA Repair 24, 63-72 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
During replication, bypass of DNA lesions is orchestrated by the Rad6 pathway. Monoubiquitination of proliferating cell nuclear antigen (PCNA) by Rad6/Rad18 leads to recruitment of translesion polymerases for direct and potentially mutagenic damage bypass. An error-free bypass pathway may be initiated via K63-linked PCNA polyubiquitination by Ubc13/Mms2 and the E3 ligase Rad5 in yeast, or HLTF/SHPRH in vertebrates. For the latter two enzymes, redundancy with a third E3 ligase and alternative functions have been reported. We have previously shown that the Rad6 pathway is involved in somatic hypermutation of immunoglobulin genes in B lymphocytes. Here, we have used knockout strategies targeting expression of the entire SHPRH protein or functionally significant domains in chicken DT40 cells that do not harbor a HLTF ortholog. We show that SHPRH is apparently redundant with another E3 ligase during DNA damage-induced PCNA modification. SHPRH plays no substantial role in cellular resistance to drugs initiating excision repair and the Rad6 pathway, but is important in survival of topoisomerase II inhibitor treatment. Removal of only the C-terminal RING domain does not interfere with this SHPRH function. SHPRH inactivation does not substantially impact on the overall efficacy of Ig diversification. Redundancy of E3 ligases in the Rad6 pathway may be linked to its different functions in genome maintenance and genetic plasticity.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Dna Repair ; Dt40 ; Pcna ; Shprh ; Ubiquitin
ISSN (print) / ISBN 1568-7864
e-ISSN 1568-7856
Journal DNA Repair
Quellenangaben Volume: 24, Issue: , Pages: 63-72 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Research Unit Gene Vector (AGV)