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Generation of targeted mouse mutants by embryo microinjection of TALENs.
Methods 69, 94-101 (2014)
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Knockin ; Knockout ; One-cell Embryo ; Gene Targeting ; Talen ; Mouse; Zinc-finger Nucleases; Double-strand Breaks; Homologous Recombination; Dna Cleavage; Effector Nucleases; Mammalian-cells; Gene Disruption; Iii Effectors; Genome; Mice
ISSN (print) / ISBN
1046-2023
e-ISSN
1095-9130
Journal
Methods
Quellenangaben
Volume: 69,
Issue: 1,
Pages: 94-101
Publisher
Elsevier
Publishing Place
Amsterdam [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)