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Pinto, S.* ; Sommermeyer, D.* ; Michel, C.* ; Wilde, S. ; Schendel, D.J. ; Uckert, W.* ; Blankenstein, T.* ; Kyewski, B.*

Misinitiation of intrathymic MART-1 transcription and biased TCR usage explain the high frequency of MART-1-specific T cells.

Eur. J. Immunol. 44, 2811-2821 (2014)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Immunity to tumor differentiation antigens, such as melanoma antigen recognized by T cells 1 (MART-1), has been comprehensively studied. Intriguingly, CD8+ T cells specific for the MART-126(27)-35 epitope in the context of HLA-A0201 are about 100 times more abundant compared with T cells specific for other tumor-associated antigens. Moreover, MART-1-specific CD8+ T cells show a highly biased usage of the Vα-region gene TRAV12-2. Here, we provide independent support for this notion, by showing that the combinatorial pairing of different TCRα- and TCRβ -chains derived from HLA-A2- MART-126-35-specific CD8+ T-cell clones is unusually permissive in conferring MART-1 specificity, provided the CDR1a TRAV12-2 region is used. Whether TCR bias alone accounts for the unusual abundance of HLA-A2-MART-126-35-specific CD8+ T cells has remained conjectural. Here, we provide an alternative explanation: misinitiated transcription of the MART-1 gene resulting in truncated mRNA isoforms leads to lack of promiscuous transcription of the MART-126-35 epitope in human medullary thymic epithelial cells and, consequently, evasion of central self-tolerance toward this epitope. Thus, biased TCR usage and leaky central tolerance might act in an independent and additive manner to confer high frequency of MART-126-35-specific CD8+ T cells.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Central Tolerance ; Human Medullary Thymic Epithelial Cells ; Mart-1 ; Melanoma ; Promiscuous Gene Expression; Peripheral-tissue Antigens; Medullary Epithelial-cells; Thymic Epithelium; Dendritic Cells; Melanoma-cells; Alpha-chain; Peptide; Expression; Tolerance; Tumor
ISSN (print) / ISBN 0014-2980
e-ISSN 1521-4141
Journal European Journal of Immunology
Quellenangaben Volume: 44, Issue: 9, Pages: 2811-2821 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)