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Rottach, A.* ; Kremmer, E. ; Nowak, D.* ; Leonhardt, H.* ; Cardoso, M.C.*

Generation and characterization of a rat monoclonal antibody specific for multiple red fluorescent proteins.

Monoclon. Antib. Immunodiagn. Immunother. 27, 337-343 (2008)
DOI
Fluorescent proteins (FP) are widely used as in vivo reporter molecules and are available in multiple colors spanning almost the entire visible light spectrum. Genetically fused to any protein target, FPs offer a powerful tool to study protein localization and dynamics. After the isolation of the prototypical green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. that provided a better spectral separation from cellular autofluorescence and allowed multicolor tracking of fusion proteins. However, the obligate tetramerization of DsRed caused serious problems for its use in live-cell imaging. Subsequent mutageneses of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc). These improved red FPs are characterized by higher brightness and photostability, complete chromophore maturation, and promise a wide variety of features for biological imaging and multicolor labeling. Here we report the generation and characterization of the first rat monoclonal antibody (MAb) against multiple red FPs, designated as multi-red 5F8. We demonstrate that multi-red 5F8 is a MAb with high affinity and specificity against the DsRed derivatives and corresponding fusion proteins, and that it is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays. Applying our versatile antibody, one and the same red fluorescent protein tag can be used to perform not only microscopic studies, but also multiple biochemical assays.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0272-457X
e-ISSN 1557-8097
Journal Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Quellenangaben Volume: 27, Issue: 5, Pages: 337-343 Article Number: , Supplement: ,
Publisher Mary Ann Liebert
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)