PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Stemberger, C.* ; Graef, P.* ; Odendahl, M.* ; Albrecht, J. ; Doessinger, G.* ; Anderl, F.* ; Buchholz, V.R.* ; Gasteiger, G. ; Schiemann, M. ; Grigoleit, G.U.* ; Schuster, F.R.* ; Borkhardt, A.* ; Versluys, B.* ; Tonn, T.* ; Seifried, E.* ; Einsele, H.* ; Germeroth, L.* ; Busch, D.H. ; Neuenhahn, M.

Lowest numbers of primary CD8+ T cells can reconstitute protective immunity upon adoptive immunotherapy.

Blood 124, 628-637 (2014)
Publ. Version/Full Text Postprint DOI PMC
Open Access Green
Patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) are threatened by potentially lethal viral manifestations like cytomegalovirus (CMV) reactivation. Because the success of today's virostatic treatment is limited by side effects and resistance development, adoptive transfer of virus-specific memory T cells derived from the stem cell donor has been proposed as an alternative therapeutic strategy. In this context, dose minimization of adoptively transferred T cells might be warranted for the avoidance of graft-versus-host disease (GVHD), in particular in prophylactic settings after T-cell-depleting allo-HSCT protocols. To establish a lower limit for successful adoptive T-cell therapy, we conducted low-dose CD8(+) T-cell transfers in the well-established murine Listeria monocytogenes (L.m.) infection model. Major histocompatibility complex-Streptamer-enriched antigen-specific CD62L(hi) but not CD62L(io) CD8(+) memory T cells proliferated, differentiated, and protected against L.m. infections after prophylactic application. Even progenies derived from a single CD62L(hi) L.m.-specific CD8(+) T cell could be protective against bacterial challenge. In analogy, low-dose transfers of Streptamer-enriched human CMV-specific CD8(+) T cells into allo-HSCT recipients led to strong pathogen-specific T-cell expansion in a compassionate-use setting. In summary, low-dose adoptive T-cell transfer (ACT) could be a promising strategy, particularly for prophylactic treatment of infectious complications after allo-HSCT.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
9.775
2.425
68
35
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Versus-host-disease; Cd8-alpha(+) Dendritic Cells; Acute Myeloid-leukemia; Listeria-monocytogenes; Complete Remission; Bone-marrow; In-vivo; Memory; Transplantation; Cytomegalovirus
Language english
Publication Year 2014
HGF-reported in Year 2014
ISSN (print) / ISBN 0006-4971
e-ISSN 1528-0020
Journal Blood
Quellenangaben Volume: 124, Issue: 4, Pages: 628-637 Article Number: , Supplement: ,
Publisher American Society of Hematology
Publishing Place Washington
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)
Institute of Virology (VIRO)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Immune Response and Infection
PSP Element(s) G-501790-003
G-520100-001
PubMed ID 24855206
DOI 10.1182/blood-2013-12547349
WOS ID WOS:000342619600026
Erfassungsdatum 2014-11-03
[➜Report correction]