PuSH - Publication Server of Helmholtz Zentrum München

Leiminger, J.H.* ; Bäßler, E.* ; Knappe, C. ; Bahnweg, G. ; Hausladen, H.*

Quantification of disease progression of Alternaria spp. on potato using real-time PCR.

Eur. J. Plant Path. 141, 295-309 (2015)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Potato early blight and brown spot are important fungal diseases responsible for premature defoliation and yield loss of potato. Pathogens considered to be involved in leaf necrosis are Alternaria solani and A. alternata, respectively. Both diseases are commonly characterized by the visualization of leaf lesions. Current detection and identification methods for Alternaria species rely primarily on cultural and morphological characteristics, the assessment of which is time-consuming and not always suitable. Sensitive, reliable methods for estimating infection severity are therefore desirable. In this study, an Alternaria-specific real-time PCR assay was developed using primers based on internal transcribed spacers (ITS) 1 and 2. The assays facilitated species detection and clearly discriminated between A. solani and A. alternata. The use of real-time PCR allowed quantitative estimation of fungal biomass in plant tissues. Detection sensitivities were in the range of >100 fg. Real-time PCR applications used to accurately assess the extent of colonization by Alternaria spp. during disease development are reported here for the first time. Additionally, Alternaria genomic DNA levels were verified not only in potato leaves showing different levels of disease progress, but also in symptomless leaves. This assay provides a useful tool to quantify pathogen levels during initial latent stages of infection and will thus help in the early detection and quantification of Alternaria spp..
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Early Blight ; Molecular Diagnosis ; Pathogen Quantification ; Qpcr ; Real-time Pcr; Blight; Solani; Strategies; Fungicides; Resistance
ISSN (print) / ISBN 0028-2944
e-ISSN 1573-8469
Journal European Journal of Plant Pathology
Quellenangaben Volume: 141, Issue: 2, Pages: 295-309 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Dordrecht
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)