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Open Access Green as soon as Postprint is submitted to ZB.
Dynamic neuroanatomy at subcellular resolution in the zebrafish.
Methods Mol. Biol. 1082, 187-195 (2014)
Genetic means to visualize and manipulate neuronal circuits in the intact animal have revolutionized neurobiology. "Dynamic neuroanatomy" defines a range of approaches aimed at quantifying the architecture or subcellular organization of neurons over time during their development, regeneration, or degeneration. A general feature of these approaches is their reliance on the optical isolation of defined neurons in toto by genetically expressing markers in one or few cells. Here we use the afferent neurons of the lateral line as an example to describe a simple method for the dynamic neuroanatomical study of axon terminals in the zebrafish by laser-scanning confocal microscopy.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Sprecher, S.G.*
Keywords
Fluorescent Protein ; Live Imaging ; Microscopy ; Neuroanatomy ; Zebrafish
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Brain Development : Methods and Protocols V
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1082,
Pages: 187-195
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed