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Grimm, M.* ; Stephan, R.* ; Iversen, C.* ; Manzardo, G.G.* ; Rattei, T.* ; Riedel, K.* ; Ruepp, A. ; Frishman, D.* ; Lehner, A.*

Cellulose as an extracellular matrix component present in Enterobacter sakazakii biofilms.

J. Food Prot. 71, 13-18 (2008)
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Open Access Green as soon as Postprint is submitted to ZB.
Cellulose was identified and characterized as an extracellular matrix component present in the biofilm of an Enterobacter sakazakii clinical isolate grown in nutrient-deficient (M9) medium. Using a bacterial artificial cloning approach in Escherichia coli and subsequent screening of transformants for fluorescence on calcofluor plates, nine genes organized in two operons were identified as putatively responsible for the biosynthesis of cellulose. In addition to the genes already described for cellulose production, two more genes were identified, putatively transcribed together with the genes from the first operon. Putative cellulose in E. sakazakii ES5 biofilm grown on glass coverslips was visualized by calcofluor staining and confocal fluorescence laser scanning microscopy. For the first time, the presence of cellulose in biofilms produced by E. sakazakii was confirmed by methylation analysis.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0362-028X
e-ISSN 1944-9097
Journal Journal of Food Protection
Quellenangaben Volume: 71, Issue: 1, Pages: 13-18 Article Number: , Supplement: ,
Publisher International Association for Food Protection
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Bioinformatics and Systems Biology (IBIS)