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Delecluse, H.J. ; Pich, D. ; Hilsendegen, T. ; Baum, C.* ; Hammerschmidt, W.

A first-generation packaging cell line for Epstein-Barr virus-derived vectors.

Proc. Natl. Acad. Sci. U.S.A. 96, 5188-5193 (1999)
DOI PMC
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
On the basis of the B lymphotropic Epstein-Barr virus (EBV), we have constructed a virus-free packaging cell line that allows encapsidation of plasmids into herpesvirus particles. This cell line harbors an EBV mutant whose packaging signals have been deleted. The gene vectors, which can encompass very large, contiguous pieces of foreign DNA, carry all cis-acting elements involved in amplification and encapsidation into virus-like particles as well as those essential for extrachromosomal maintenance in the recipient cell. Although this first-generation packaging cell line suffers from unwanted recombination between the helper virus genome and gene vector DNAs, this approach opens the way to delivery and stable maintenance of any transgene in human B cells.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 96, Issue: 9, Pages: 5188-5193 Article Number: , Supplement: ,
Publisher National Academy of Sciences
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)