PuSH - Publication Server of Helmholtz Zentrum München: Metabonomics study on the effects of the ginsenoside Rg3 in a beta-cyclodextrin-based formulation on tumor-bearing rats by a fully automatic hydrophilic interaction/reversed-phase column-switching HPLC-ESI-MS approach.

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Wang, Y.* ; Wang, J.* ; Yao, M.* ; Zhao, X.* ; Fritsche, J.* ; Schmitt-Kopplin, P. ; Cai, Z.* ; Wan, D.* ; Lu, X.* ; Yang, S.* ; Gu, J.* ; Häring, H.-U.* ; Schleicher, E.D.* ; Lehmann, R.* ; Xu, G.*

Metabonomics study on the effects of the ginsenoside Rg3 in a beta-cyclodextrin-based formulation on tumor-bearing rats by a fully automatic hydrophilic interaction/reversed-phase column-switching HPLC-ESI-MS approach.

Anal. Chem. 80, 4680-4688 (2008)

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Open Access Green as soon as Postprint is submitted to ZB.

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The goal of this study was the application of a novel, fully automatic column-switching approach in a metabonomics study combining the orthogonal selectivities of hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. The temporal, pharmacodynamic effects of the ginsenoside Rg3 on the metabonome in urine of healthy and liver-tumor-bearing rats have been investigated. Within a total analysis time of 52 min we detected 5686 polar, and on the second column an additional 1808 apolar, urinary metabolite ions. The administration of a single, high dose of Rg3 in a beta-cyclodextrin-based formulation led to a considerable change of the metabolic pattern in cancer rats during 3 days studied. Seventeen biomarker candidates including three apolar metabolites, which were not retained on the HILIC column, were detected. Overall, the results suggest that the developed liquid chromatography-mass spectrometry strategy is a promising tool in metabonomics studies for global analysis of highly complex biosamples. It may not only increase the number of discovered biomarkers but consequently improve the comprehensive information on metabolic changes in a fully automatic manner.

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Publication type Article: Journal article

Document type Scientific Article

Keywords metabonomics; HPLC-ESI-MS; hydrophilic interaction chromatography (HILIC); reversed-phase chromatography; biomarkers

ISSN (print) / ISBN 0003-2700

e-ISSN 1520-6882

Journal Analytical Chemistry

Quellenangaben Volume: 80, Issue: 12, Pages: 4680-4688

Publisher American Chemical Society (ACS)

Reviewing status Peer reviewed

Institute(s) Institute of Ecological Chemistry (IOEC)