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Open Access Green as soon as Postprint is submitted to ZB.
HLA-class II antigens on hemopoietic and stromal cells in human micro long-term bone marrow cultures.
Exp. Hematol. 18, 103-108 (1990)
Using a complement-dependent cytotoxicity assay (CDC), we analyzed the presence of HLA-class II antigens on both stromal and hemopoietic cells in a miniaturized human long-term bone marrow culture system. 4-Hydroperoxycyclophosphamide (4-HC)-resistant hemopoietic stem cells capable of restoring in vitro hemopoiesis on irradiated stromal cell layers were HLA-DR, -DP, and -DQ negative. In addition, these cells failed to bind the monoclonal antibody (mAb) Tu 39, previously proposed as a candidate for the recognition of a novel class II antigen, '-DY'. On the other hand, the formation of confluent stromal cell layers was inhibited by HLA-DR- or -DP-specific mAbs, but not by the HLA-DQ-specific mAb Tu 22. This suggests the presence of HLA-DR- and/or HLA-DP-positive, but HLA-DQ-negative stromal precursor cells.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Hemopoietic Stem Cells ; Hla Antigens ; Long-term Bone Marrow Culture ; Monoclonal Antibodies ; Stromal Cells
ISSN (print) / ISBN
0301-472X
e-ISSN
0301-472X
Journal
Experimental Hematology
Quellenangaben
Volume: 18,
Issue: 2,
Pages: 103-108
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Experimentelle Hämatologie