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Wagner, T. ; Arango, I.L.M. ; Grundmann, S. ; Dörfler, U. ; Schroll, R. ; Schloter, M. ; Hartmann, A. ; Sandermann, H. ; Ernst, D.

The probability of a horizontal gene transfer from Roundup Ready soybean to root symbiotic bacteria: A risk assessment study on the GSF lysimeter station.

Water Air Soil Pollut. 8, 155-162 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The gene transfer from glyphosate tolerant soybean to Bradyrhizobium japonicum was evaluated in a free-air lysimeter experiment under natural conditions and increasing selection pressure, to monitor for the probability of horizontal gene transfer (HGT). A large volume lysimeter study that offers conditions comparable to normal farming was conducted in 2004 and 2005 with Roundup Ready® (RR) soybean and Roundup® application according to agricultural practice. Analysis of nodules showed, as expected, the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). However, in bacteroids that were isolated from nodules and then cultivated for several rounds in the presence of high levels of glyphosate, the EPSPS gene could no longer be detected. This indicates no stable HGT transfer of the whole EPSPS gene under field conditions. © 2007 Springer Science+Business Media B.V.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Enolpyruvylshikimate-3-phosphate synthase; free-air lysimeter; glyphosate; horizontal gene transfer; Roundup Ready soybean; symbiotic bacteria
ISSN (print) / ISBN 0049-6979
e-ISSN 1573-2932
Journal Water, Air, & Soil Pollution
Quellenangaben Volume: 8, Issue: 2, Pages: 155-162 Article Number: , Supplement: ,
Publisher Springer
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
Institute of Soil Ecology (IBOE)
Research Unit Microbe-Plant Interactions (AMP)