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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
A simple effective procedure for the determination of adenosine triphosphate in soils.
Chemosphere 17, 2461-2470 (1988)
A simple method for the extraction and determination of adenosine 5′-triphosphate (ATP) in soil is described. ATP was extracted by stirring with dimethyl sulphoxide (DMSO), followed by adding 0.01 M trisodium phosphate (Na3PO4) buffer solution for a further dispersion. The ATP content was quantitatively measured by luciferin-luciferase system employing a standard addition technique. The method was most efficient in comparison with 7 other extraction procedures. The recoveries of ATP in spiked soil were found to be approximately 100 %. ATP contents in 16 selected soils were in ranges of 0.76±0.05 - 7.79±0.83 μg/g (dry weight). A significant correlation between ATP amounts and biomasses in these soils was also observed.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0045-6535
e-ISSN
1879-1298
Journal
Chemosphere
Quellenangaben
Volume: 17,
Issue: 12,
Pages: 2461-2470
Publisher
Elsevier
Publishing Place
Kidlington, Oxford
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)