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Open Access Green as soon as Postprint is submitted to ZB.
Exploration of intraclonal adaptation mechanisms of Pseudomonas brassicacearum facing cadmium toxicity.
Environ. Microbiol. 9, 2820-2835 (2007)
Pseudomonas brassicacearum forms phenotypic variants in vitro as well as in planta during root colonization under natural conditions, leading to subpopulations (phase I and II cells) that differ in colony morphology and production of exoenzymes/secondary metabolites. The maximal concentration of cadmium allowing both variants growth was 25 muM; however, phase II cells accumulated fivefold higher Cd than phase I cells, even though both variants showed the same growth rate and kinetics, comprising a long stasis period (50 h). The whole transcriptome analysis of both variants in response to Cd was investigated using the home-made DNA microarrays. This analysis revealed completely different adaptation mechanisms developed by each variant to withstand and grow in the presence of the toxic. A re-organization of the cell wall to limit Cd entrance was noticed for phase I cells, as genes encoding levan exopolymers were downregulated at the expense of an upregulation of genes encoding alginate, and an upregulation of transporters such as cadA, and a downregulation of copper transporters. Phase II cells were unable to prevent Cd entrance and recruited genes under the control of oxyR and soxR regulation to face osmotic and oxidant stresses generated by Cd. Putrescine and spermidine metabolism appeared to play a central role in Cd tolerance. Microarray data were validated by biological analyses such as motility, oxidative stress assay, metabolite profiling with ICR-FT/MS and UPLC, capillary electrophoresis analysis of biogenic amines.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Pseudomonas brassicacearum; cadmium toxicity; microarray; ICR-FT/MS; UPLC; metabolite profiling
ISSN (print) / ISBN
1462-2912
e-ISSN
1462-2920
Journal
Environmental Microbiology
Quellenangaben
Volume: 9,
Issue: 11,
Pages: 2820-2835
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)