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Activation-induced cytidine deaminase initiates immunoglobulin gene conversion and hypermutation by a common intermediate.
PLoS Biol. 2, 967-974:e179 (2004)
Depending on the species and the lymphoid organ, activation-induced cytidine deaminase (AID) expression triggers diversification of the rearranged immunoglobulin (Ig) genes by pseudo V (psiV) gene- templated gene conversion or somatic hypermutation. To investigate how AID can alternatively induce recombination or hypermutation, psiV gene deletions were introduced into the rearranged light chain locus of the DT40 B-cell line. We show that the stepwise removal of the psiV donors not only reduces and eventually abolishes Ig gene conversion, but also activates AID-dependent Ig hypermutation. This strongly supports a model in which AID induces a common modification in the rearranged V(D)J segment, leading to a conversion tract in the presence of nearby donor sequences and to a point mutation in their absence.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
CLASS SWITCH RECOMBINATION; DT40 CELL-LINE; B-CELLS; SOMATIC HYPERMUTATION; GERMINAL-CENTERS; AID; REQUIREMENT; REPERTOIRE; MUTANT; ENZYME
ISSN (print) / ISBN
1544-9173
e-ISSN
1545-7885
Journal
PLoS Biology
Quellenangaben
Volume: 2,
Issue: 7,
Pages: 967-974,
Article Number: e179
Publisher
Public Library of Science (PLoS)
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Radiation Biology (IMS)