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Markoullis, K. ; Bulian, D. ; Hölzlwimmer, G. ; Quintanilla-Martinez, L. ; Heiliger, K.-J. ; Zitzelsberger, H. ; Scherb, H. ; Mysliwietz, J. ; Uphoff, C.C.* ; Drexler, H.G.* ; Thure, A.* ; Adler, T. ; Busch, D.H. ; Schmidt, J. ; Mahabir, E.

Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny.

Transgenic Res. 18, 71-87 (2009)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Murine embryonic stem cells (mESCs) inoculated at passage P13 with the mycoplasma species M. hominis, M. fermentans and M. orale and cultured over 20 passages showed reduced growth rate and viability (P < 0.0001) compared to control mESCs. Spectral karyotypic analysis of mycoplasma-infected mESCs showed a number of non-clonal chromosomal aberrations which increased with the duration of infection. The differentiation status of the infected mESCs was most affected at passage P13+6 where the infection was strongest and 46.3% of the mESCs expressed both POU5F1 and SSEA-1 markers whereas 84.8% of control mESCs expressed both markers. The percentage of germline chimeras from mycoplasma-infected mESCs was examined after blastocyst injection and embryo transfer to suitable recipients at different passages and, compared to the respective control group, was most affected at passage P13+5 (50% vs. 90%; P < 0.07). Further reductions were obtained at the same passage in the percentage of litters born (50% vs. 100%; P < 0.07) and in the percentage of pups born (22% vs. 45%; P < 0.001). Thirty three chimeras (39.8%) obtained from blastocyst injection with mycoplasma-infected mESCs showed reduced body weight (P < 0.0001), nasal discharge, osteoarthropathia, and cachexia. Flow cytometric analysis of plasma from chimeras produced with mycoplasma-infected mESCs revealed statistically significant differences in the proportions of T-cells and increased levels of IgG1 (P < 0.001), IgG2a (P < 0.05) and IgM (P < 0.05), anti-DNA antibodies (P < 0.05) and rheumatoid factor (P < 0.01). The present data indicate that mycoplasma contamination of mESCs affects various cell parameters, germline transmission, and postnatal development of the resulting chimeras.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Murine embryonic stem cells; Mycoplasmas; Germline transmission; Chimeras
ISSN (print) / ISBN 0962-8819
e-ISSN 1573-9368
Journal Transgenic Research
Quellenangaben Volume: 18, Issue: 1, Pages: 71-87 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Radiation Biology (IMS)
CF Comparative Medicine (CF-Comparative Medicine)
Institute of Pathology (PATH)
Institute of Biomathematics and Biometry (IBB)
Institute of Molecular Immunology (IMI)
Institute of Virology (VIRO)