Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques.
FEMS Microbiol. Ecol. 71, 237-246 (2010)
A toluene-degrading microbial consortium was enriched directly in a BTEX-contaminated aquifer under sulfate-reducing conditions using in situ microcosms consisting of toluene-loaded activated carbon pellets. Degradation of toluene and concomitant sulfide production by the consortium was subsequently demonstrated in laboratory microcosms. The consortium was physiologically and phylogenetically characterized by isotope tracer experiments using nonlabeled toluene, [13C]-alpha-toluene or [13C(7)]-toluene as growth substrates. Cells incubated with [13C]-alpha-toluene or [13C(7)]-toluene incorporated 8-15 at.% 13C and 51-57 at.% 13C into total lipid fatty acids, respectively, indicating a lower specific incorporation of 13C from [13C(7)]-toluene. In order to identify the toluene-assimilating bacteria, the incorporation of carbon from both [13C]-alpha-toluene and [13C(7)]-toluene into rRNA was analyzed by stable isotope probing. Time and buoyant density-resolved 16S rRNA gene-based terminal restriction fragment length polymorphism profiles, combined with cloning and sequencing, revealed that an uncultured bacterium (99% sequence similarity) related to the genus Desulfocapsa was the main toluene-degrading organism in the consortium. The ratio of the respective terminal restriction fragments changed over time, indicating trophic interactions within this consortium.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Toluene; Stable isotope probing (SIP); In situ microcosm; Sulfate-reducing; Biodegradation
ISSN (print) / ISBN
0168-6496
e-ISSN
1574-6941
Journal
FEMS Microbiology Ecology
Quellenangaben
Volume: 71,
Issue: 2,
Pages: 237-246
Publisher
Wiley
Publishing Place
Oxford
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)