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The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing.
Nucleic Acids Res. 35, 789-800 (2007)
The nucleolar protein Pes1 interacts with Bop1 and WDR12 in a stable complex (PeBoW-complex) and its expression is tightly associated with cell proliferation. The yeast homologue Nop7p (Yph1p) functions in both, rRNA processing and cell cycle progression. The presence of a BRCT-domain (BRCA1 C-terminal) within Pes1 is quite unique for an rRNA processing factor, as this domain is normally found in factors involved in DNA-damage or repair pathways. Thus, the function of the BRCT-domain in Pes1 remains elusive. We established a conditional siRNA-based knock-down-knock-in system and analysed a panel of Pes1 truncation mutants for their functionality in ribosome synthesis in the absence of endogenous Pes1. Deletion of the BRCT-domain or single point mutations of highly conserved residues caused diffuse nucleoplasmic distribution and failure to replace endogenous Pes1 in rRNA processing. Further, the BRCT-mutants of Pes1 were less stable and not incorporated into the PeBoW-complex. Hence, the integrity of the BRCT-domain of Pes1 is crucial for nucleolar localization and its function in rRNA processing.
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35
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
FACTOR TIF-IA; CELL-PROLIFERATION; PROTEIN L11; DNA-DAMAGE; BIOGENESIS; CYCLE; P53; PESCADILLO; COMPLEX; STRESS
Language
english
Publication Year
2007
HGF-reported in Year
0
ISSN (print) / ISBN
0305-1048
e-ISSN
1362-4962
Journal
Nucleic Acids Research
Quellenangaben
Volume: 35,
Issue: 3,
Pages: 789-800
Publisher
Oxford University Press
Reviewing status
Peer reviewed
Institute(s)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Molecular Immunology (IMI)
Institute of Molecular Immunology (IMI)
POF-Topic(s)
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s)
Immune Response and Infection
PSP Element(s)
G-501400-001
G-501700-003
G-501700-003
PubMed ID
17189298
WOS ID
000244429800014
Scopus ID
33847365771
Erfassungsdatum
2007-07-10