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Rosenberg, K.* ; Bertaux, J.* ; Krome, K.* ; Hartmann, A. ; Scheu, S.* ; Bonkowski, M.*

Soil amoebae rapidly change bacterial community composition in the rhizosphere of Arabidopsis thaliana.

ISME J. 3, 675-684 (2009)

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Open Access Green as soon as Postprint is submitted to ZB.

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We constructed an experimental model system to study the effects of grazing by a common soil amoeba, Acanthamoeba castellanii, on the composition of bacterial communities in the rhizosphere of Arabidopsis thaliana. Amoebae showed distinct grazing preferences for specific bacterial taxa, which were rapidly replaced by grazing tolerant taxa in a highly reproducible way. The relative proportion of active bacteria increased although bacterial abundance was strongly decreased by amoebae. Specific bacterial taxa had disappeared already two days after inoculation of amoebae. The decrease in numbers was most pronounced in Betaproteobacteria and Firmicutes. In contrast, Actinobacteria, Nitrospira, Verrucomicrobia and Planctomycetes increased. Although other groups, such as betaproteobacterial ammonia oxidizers and Gammaproteobacteria did not change in abundance, denaturing gradient gel electrophoresis with specific primers for pseudomonads (Gammaproteobacteria) revealed both specific changes in community composition as well as shifts in functional genes (gacA) involved in bacterial defence responses. The resulting positive feedback on plant growth in the amoeba treatment confirms that bacterial grazers play a dominant role in structuring bacteria-plant interactions. This is the first detailed study documenting how rapidly protozoan grazers induce shifts in rhizosphere bacterial community composition.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Acanthamoeba; Arabidopsis; rhizosphere; bacterial diversity; DGGE; FISH; targeted oligonucleotide probes; gram-positive bacteria; 16s ribosomal-rna; dna g+c content; plant-growth; feeding nematodes; pseudomonas-fluorescens; microbial community; faunal interactions; root architecture

ISSN (print) / ISBN 1751-7362

e-ISSN 1751-7370

Journal ISME Journal

Quellenangaben Volume: 3, Issue: 6, Pages: 675-684

Publisher Nature Publishing Group

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Microbe-Plant Interactions (AMP)