PuSH - Publication Server of Helmholtz Zentrum München

Weissinger, E.M. ; Henderson, D.W.* ; Mischak, H. ; Goodnight, J.* ; Frederic Mushinski, J.*

Induction of plasmacytomas that secrete monoclonal anti-peptide antibodies by retroviral transformation.

J. Immunol. Methods 168, 123-130 (1994)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
ABL-MYC, a retrovirus that coexpresses v-abl and c-myc, was used to infect six BALB/c mice that had been immunized twice with a KLH-conjugated peptide that consisted of the 18 carboxyterminal amino acids of protein kinase C-η (PKC-η). All mice developed transplantable, monoclonal plasmacytomas, and five out of six plasmacytomas secreted antigen-specific antibodies, even after transplantation. All these antibodies recognized PKC-η on Western blots of crude cell lysates and did not cross react with other isoforms of the PKC family.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Abl-myc ; Monoclonal Antibody ; Proteinkinase C-η ; Retrovirus
ISSN (print) / ISBN 0022-1759
e-ISSN 1872-7905
Journal Journal of Immunological Methods
Quellenangaben Volume: 168, Issue: 1, Pages: 123-130 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)