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Open Access Green as soon as Postprint is submitted to ZB.
The effect of the rat immunoglobulin heavy-chain 3' enhancer is position dependent.
Gene 136, 349-353 (1993)
We tested the effect of the immunoglobulin (Ig) heavy- and κ-chain 3' enhancers on the expression of Ig genes in B-cells. Inclusion of the heavy-chain 3' enhancer in addition to the μ intron enhancer increased the expression rate up to sixfold, but this effect was strongly position dependent, in that it was only observed when the element was located downstream from the constant exons. Furthermore, the stimulatory effect could be augmented by increasing the distance between the constant gene segments and the 3' enhancer. When the 3' enhancer was located upstream from the variable gene promoter, the transcription was dramatically suppressed. Thus, the heavy-chain 3' enhancer does not fit into the usual definition of an enhancer element. The implications for the production of recombinant antibodies are discussed.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Electroporation ; Expression Vector ; Myeloma Cell ; Recombinant Antibody ; Transcriptional Regulation
ISSN (print) / ISBN
0378-1119
e-ISSN
1879-0038
Journal
Gene
Quellenangaben
Volume: 136,
Issue: 1-2,
Pages: 349-353
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Immunologie