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Ozone induction and purification of spruce cinnamyl alcohol dehydrogenase.

Phytochemistry 32, 557-563 (1993)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Spruce needle cinnamyl alcohol dehydrogenase (CAD) was induced in seedlings and four-year-old trees two-to-four-fold by ozone concentrations that did not lead to visible damage. CAD thus acted as an early biochemical ozone marker. A highly purified CAD preparation consisting of two ∼42 000 Mr subunits was prepared from spruce cell cultures. A polyclonal antibody was strongly inhibitory. The enzyme had high specificity for coniferyl alcohol and NADP+, and was also detected in spruce seedlings and spruce cambial sap.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cinnamyl Alcohol Dehydrogenase ; Lignin Biosynthesis ; Ozone. ; Picea Abies ; Pinaceae ; Spruce
ISSN (print) / ISBN 0031-9422
e-ISSN 1873-3700
Journal Phytochemistry
Quellenangaben Volume: 32, Issue: 3, Pages: 557-563 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed