PuSH - Publication Server of Helmholtz Zentrum München: Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal scanning laser microscopy.

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Schloter, M. ; Borlinghaus, R.T. ; Bode, W. ; Hartmann, A.

Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal scanning laser microscopy.

J. Microsc. 171, 173-177 (1993)

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Open Access Green as soon as Postprint is submitted to ZB.

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Azospirillum is a soil bacterium with plant growth-promoting potential. Strain-specific monoclonal antibodies (mAbs) which bind to different antigenic determinants of the bacterial cell surface with high specificity for A. brasilense Sp7 were used to monitor this bacterium in the rhizosphere of wheat plants. The mAbs were marked directly with the fluorochromes fluorescein (FITC) or tetramethylrhodamine (TRITC). With the labelled mAbs and focal scanning laser microscopy, an in situ identification and localization of this bacterium in root segments was possible.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Azospirillum ; Confocal Scanning Laser Microscopy ; Monoclonal Antibodies

ISSN (print) / ISBN 0022-2720

e-ISSN 1365-2818

Journal Journal of Microscopy

Quellenangaben Volume: 171, Issue: 2, Pages: 173-177

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Soil Ecology (IBOE)
Research Unit Microbe-Plant Interactions (AMP)