PuSH - Publication Server of Helmholtz Zentrum München: Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco.

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Neininger, A.* ; Bichler, J.* ; Schneiderbauer, A. ; Mohr, H.J.*

Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco.

Planta 189, 440-442 (1993)

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Open Access Green as soon as Postprint is submitted to ZB.

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In the present study the question was addressed of whether the nitrite-reductase (NIR-)promoter from spinach (Spinacia oleracea L.), fused to a reporter gene (bacterial β-glucuronidase, GUS) and introduced into tobacco (Nicotiana tabacum L.) responds to nitrate and light in accordance with spinach (donor) or in accordance with tobacco (host). The data obtained at the GUS enzyme level as well as at the transcript level allow an unambiguous answer to this question: GUS gene expression under the control of the NIR-promoter from spinach responds to nitrate and light in accordance with the host (tobacco). Expression of the promoter-less GUS gene was not induced by any treatment.

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Publication type Article: Journal article

Document type Scientific Article

Keywords Gene Expression ; Nicotiana ; Nitrate ; Nitrite Reductase ; Phytochrome ; Transgenic Tobacco

ISSN (print) / ISBN 0032-0935

e-ISSN 1432-2048

Journal Planta

Quellenangaben Volume: 189, Issue: 3, Pages: 440-442

Publisher Springer

Reviewing status Peer reviewed

Institute(s) Institute of Biochemical Plant Pathology (BIOP)