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Open Access Green as soon as Postprint is submitted to ZB.
Large scale production and purification of human retrovirus-like particles related to the mouse mammary tumor virus.
FEMS Microbiol. Lett. 109, 289-296 (1993)
Human retrovirus-like particles related to mouse mammary tumor virus (MMTV) are secreted in a steroid-dependent manner by the breast cancer cell line T47D. We report the successful large scale production and purification of these particles from culture supernatants of T47D cells and describe the experimental conditions established for this purpose. Thus, mg amounts of particles were produced by large scale culturing of T47D cells in an autoharvesting roller bottle system and purified by differential centrifugation and continuous flow ultracentrifugation on density gradients with a 50% recovery and a 350-fold enrichment.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Human Mmtv-related Particles ; Large Scale Purification
ISSN (print) / ISBN
0378-1097
e-ISSN
1574-6968
Journal
FEMS Microbiology Letters
Quellenangaben
Volume: 109,
Issue: 2-3,
Pages: 289-296
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)