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Apel, T.W. ; Mautner, J.M. ; Polack, A. ; Bornkamm, G.W. ; Eick, D.

Two antisense promoters in the immunoglobulin μ-switch region drive expression of c-myc in the Burkitt's lymphoma cell line BL67.

Oncogene 7, 1267-1271 (1992)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
In the Burkitt's lymphoma (BL) cell line BL67 the first exon of the c-myc gene is fused to the μ-switch region of the immunoglobulin heavy-chain gene (IgH). BL67 cells express IgH/c-myc hybrid RNAs which are initiated in the immunoglobulin locus, transcribed across the chromosomal breakpoint into the first exon of c-myc and spliced using the physiological splice donor and acceptor sites of the c-myc gene. We have isolated cDNAs of these hybrid RNAs and characterized the start points in the Ig heavy-chain gene. Two promoters were identified in the μ-switch region of BL67 cells which give rise to antisense transcription of the μ-gene. These promoters are also active in other BL cell lines, in B cells without Ig translocation and in a T-cell line. Both promoters co-localize with DNAase I-hypersensitive sites, HNF and HSW, in the μ-switch region. The structures of IgH/c-myc hybrid RNAs and of the corresponding promoters are described.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0950-9232
e-ISSN 0950-9232
Journal Oncogene
Quellenangaben Volume: 7, Issue: 7, Pages: 1267-1271 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)