PuSH - Publication Server of Helmholtz Zentrum München: Coupled-column high-performance liquid chromatographic method for the determination of 1-hydroxypyrene in urine of subjects exposed to polycyclic aromatic hydrocarbons.

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Boos, K.S.* ; Lintelmann, J. ; Kettrup, A.

Coupled-column high-performance liquid chromatographic method for the determination of 1-hydroxypyrene in urine of subjects exposed to polycyclic aromatic hydrocarbons.

J. Chromatogr. A 600, 189-194 (1992)

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A coupled-column high-performance liquid chromatographic system for integrated, on-line sample processing and the determination of free and conjugated 1-hydroxypyrene in urine has been developed. The method is based on a "tailor-made" copper phthalocyanine-modified porous-glass precolumn packing material, which allows a direct and repeated injection of urine samples and a selective enrichment of trace amounts of particular components. The fully automated method has a low detection limit (0.01 pmol), a quantitative and matrix-independent recovery and a highly reliability, as shown by an interlaboratory comparison of methods.

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