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The REV2 gene of Saccharomyces cerevisiae: Cloning and DNA sequence.
Curr. Genet. 22, 277-282 (1992)
The REV2 gene of Saccharomyces cerevisiae was cloned and sequenced; it contains an open reading frame of 1986 bp with a coding potential of 662 amino acids. Interruption of the chromosomal REV2 gene by integrating the URA3 gene coupled with partial deletion of the 3' terminal region produced viable haploid rev2Δ mutants. This indicates that the REV2 gene is non-essential for growth. The rev2Δ mutant is slightly more UV-sensitive than strains carrying various rev2 alleles (rev2-1, rev2x, rad5-1, rad5-8). The putative Rev2 protein is probably a globular protein containing a highly conserved nucleotide-binding site and two zinc-finger domains.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Dna-repair ; Mutation-deficient Mutant ; Nucleotide-binding Consensus ; Yeast
ISSN (print) / ISBN
0172-8083
e-ISSN
1432-0983
Journal
Current Genetics
Quellenangaben
Volume: 22,
Issue: 4,
Pages: 277-282
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)