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Open Access Green as soon as Postprint is submitted to ZB.
Differential staining of human and murine chromatin in situ by hybridization with species-specific satellite DNA probes.
Biochem. Biophys. Res. Commun. 182, 1313-1319 (1992)
Human and murine chromatin was differentially labeled by hybridization with DNA probes that bind to species-specific satellite DNA. The targets for in situ hybridization were the mouse-specific major or gamma satellite DNA and the human alpha satellite DNA. These sequences typically are localized at or near the chromosome centromeres, and remain their tight localization throughout the cell cycle. DNA probes were synthesized in vitro by primer directed DNA amplification using the polymerase chain reaction. In typical applications like the differentiation of cells derived from chimeric animals or the characterization of chromosomes in somatic cell hybrids, the two DNA probes are differently labeled and detected using label-specific reagents that fluoresce at different wavelengths. The rapid technique for chromatin discrimination described here combines high specificity with unprecedented signal intensity.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0006-291X
e-ISSN
1090-2104
Quellenangaben
Volume: 182,
Issue: 3,
Pages: 1313-1319
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Abteilung für Strahlenbiologie und Biophysik