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Open Access Green as soon as Postprint is submitted to ZB.
Advanced quality control for zinc speciation investigations in human breast milk.
Fresen. J. Anal. Chem. 344, 306-310 (1992)
Information is provided about the reliability of results obtained in Zn-speciation analyses of human milk. For such analyses, convenient techniques have already been given in an earlier paper [1], comprising mass balances of metal and ligand, purity of analytes, etc. Here, however, the influence of the eluent was checked in an extended manner. For this series of experiments, bi-distilled water was compared with different buffers as the eluent. Water proved to be a more suitable mobile phase than buffers with regard to contamination. Moreover, a method was developed to investigate the possibility of a transfer of Zn among the proteins during size exclusion chromatography. For these experiments, casein and metallothionein were chosen as competitive Zn-ligands, showing extremely different affinities to Zn. A possible Zn-transfer from one protein to the other was examined in combining a Zn-containing protein with a Zn-free one. No change of the Zn-status of the proteins was detected, indicating a stable protein-metal complex under the experimental conditions. Zn and protein mass balances (injected/eluted) were calculated and found to be 100%.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0937-0633
e-ISSN
1432-1130
Quellenangaben
Volume: 344,
Issue: 7-8,
Pages: 306-310
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)