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Open Access Green as soon as Postprint is submitted to ZB.
Oxidative stress in vivo and in vitro: Modulation by quartz dust and hyperbaric atmosphere.
Free Radical Biol. Med. 12, 1-10 (1992)
Changes in the oxidative status in the soluble proteins of bronchoalveolar lavage (BAL) fluid from monkeys were examined during 26 months of individual or combined exposure to quartz dust (5 mg/m3 of DQ12) and a hyperbaric atmosphere (2.5 bar). The oxidation of BAL proteins, assumed to be an indicator for oxidative stress in the lungs, was determined by measuring the amount of carbonyl groups in their amino acid side chains. The carbonyl content of BAL proteins (nmol carbonyl/mg protein) increased steadily to a maximum value of 156% of the control after 6 months exposure to hyperbaric atmosphere, and decreased below 50% of control levels in both the quartz alone exposed group and the group exposed to quartz in combination with a hyperbaric atmosphere. The effect of quartz on the production of reactive oxygen species by BAL cells was investigated in vitro. BAL cells from healthy monkeys preincubated with quartz and stimulated with phorbolmyristate acetate (PMA) produced reduced levels of extracellular superoxide anion and intracellular hydrogen peroxide compared with PMA-only stimulated cells. Thus the lowered carbonyl content of BAL proteins in the quartz exposed groups may have resulted from reduced production of the hydrogen peroxide which is essential for carbonyl formation by phagocytes. Changes in carbonyl content of BAL protein in vivo may be a new indicator for potential subsequent lung damage.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Bal ; Bal Proteins ; Bronchoalveolar Lavage ; Free Radicals ; Hyperbaric Atmosphere ; Monkey ; Protein Oxidation ; Quartz
ISSN (print) / ISBN
0891-5849
e-ISSN
1873-4596
Quellenangaben
Volume: 12,
Issue: 1,
Pages: 1-10
Publisher
Elsevier
Publishing Place
New York, NY
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Protection (ISS)