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Open Access Green as soon as Postprint is submitted to ZB.
Radical effects on mutation spectra in lambda phage.
Int. J. Radiat. Biol. 62, 3-8 (1992)
Mutations in the lambda repressor gene cI (710 bp) were induced by 60Co-gamma radiation in dissolved lambda phage DNA. After in vitro DNA packaging to lambda phage particles (pack phage) and phenotypic expression of the mutants, DNA was sequenced directly. Two-thirds of mutations were located in the amino terminus region of the gene without any signs of hotspots. Changes consisted of (+1) insertions (25%) and base substitutions (75%). Transitions were exclusively G/C to A/T. Transversions were mostly G/C to C/G and few G/C to T/A. We did not find A/T to T/A transversions, A/T to G/C transitions, deletions and gross rearrangements. In most of the base substitutions a pre-existing base pair had been replaced by an A/T pair; this might come from non-instructional sites like abasic sites. Several mechanisms for base substitutions are considered.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0955-3002
e-ISSN
1362-3095
Quellenangaben
Volume: 62,
Issue: 1,
Pages: 3-8
Publisher
Informa Healthcare
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)