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Schott, K. ; Brand, K.A. ; Hatakeyama, K. ; Kagamiyama, H. ; Maier, J. ; Werner, T. ; Ziegler, I.

Control of cell-cycle-associated tetrahydrobiopterin synthesis in rat thymocytes.

Exp. Cell Res. 200, 105-109 (1992)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The cell-cycle progression of rat thymocytes from G0 through G1 to DNA synthesis is associated with a transient synthesis of H4biopterin, the concentration of which reaches a maximum at the time of S-phase entry and then decreases. This synthesis of H4biopterin is controlled by the specific activity of GTP cyclohydrolase I, which peaks in G1/S cells. In contrast, the catalytic activity of sepiapterin reductase remains constant throughout the cell-cycle. At G0 the steady state mRNA levels specific for GTP cyclohydrolase I and sepiapterin reductase, respectively, are below the limits of detection. Both accumulate as the thymocytes progress through the cell-cycle but lack cyclic down regulation. The data indicate that the variations in H4biopterin synthesis during the cell-cycle are caused by growth regulated increase in GTP cyclohydrolase I mRNA expression, with subsequent post-translational inactivation. This latter is likely due to the degree of enzyme phosphorylation.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1090-2422
e-ISSN 0014-4827
Journal Experimental Cell Research
Quellenangaben Volume: 200, Issue: 1, Pages: 105-109 Article Number: , Supplement: ,
Publisher Academic Press
Publishing Place Orlando, Fla.
Non-patent literature Publications
Reviewing status Peer reviewed