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Dörr, W. ; Kummermehr, J.C.

Proliferation kinetics of mouse tongue epithelium under normal conditions and following single dose irradiation.

Virchows Arch. 60, 287-294 (1991)
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Epithelial proliferation in the ventral surface of mouse tongue follows a pronounced circadian rhythm with a peak in mitotic activity at 10.00 a.m., preceded by a wave of DNA synthesis 8 h earlier. Nearly all cells (85%) pass through G2 and mitosis immediately after the S-phase; they subsequently divide again, usually after 2 or 3 days, indicating cohorts of cells with different G1-duration. The fraction of all nucleated cells comprised in one daily proliferation wave is about 20% indicating a turnover time of the nucleated cell compartment of about 5 days. Cytotoxic injury by a single radiation dose of 20 Gy causes a steep decrease in cell counts, leading to complete denudation after 9-13 days. The difference between the latent period before ulceration and the tissue turnover time is explained by a marked proliferative activity of the doomed cells. The mitotic index increases steeply after day 1 to three times the control level, but most mitotic figures display gross abnormalities such as multipolar spindles or chromosome clumping. As a consequence cells with abnormal or multiple nuclei appear in the basal layers 3 days post irradiation and subsequently migrate to the upper layers. After denudation the epithelium rapidly becomes restored, with a phase of transient hyperplasia on days 13-14. Normal architecture is regained by day 15. Over the whole healing period the mitotic index remains at a high level, with most of the mitoses appearing histologically normal.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cell Kinetics ; Circadian Rhythm ; Irradiation ; Mouse Tongue Mucosa
ISSN (print) / ISBN 0042-6423
e-ISSN 1432-2307
Journal Virchows Archiv
Quellenangaben Volume: 60, Issue: 5, Pages: 287-294 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Abteilung für Strahlenbiologie und Biophysik