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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Immunological characterization of canine hematopoietic progenitor cells.
Ann. Hematol. 63, 223-226 (1991)
Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P1 and immunomagnetic bead separation. It was regularly enriched when MdT-P1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P1-positive fraction.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Autologous Bone Marrow Transplantation ; Cfu-gm ; Dog ; Hematopoietic Progenitor Cells ; Monoclonal Antibodies
ISSN (print) / ISBN
0939-5555
e-ISSN
1432-0584
Journal
Annals of Hematology
Quellenangaben
Volume: 63,
Issue: 4,
Pages: 223-226
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institut für Immunologie